Simple and Rapid Method for Rat Estrous Cycle Identification Using Crystal Violet- Hormonal Consideration

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Nazia Begum
Kandavalli Manipriya
Rahathunnisa Begum
Veeresh B

Abstract

Rat estrous cycle determination or vaginal smear staining is paramount for studies related to endocrinology
and reproduction; in the present study; we have reported a simple and rapid method for estrous cycle determination
in rats using crystal violet. With this technique, the identification of stages can be done even on the
next day. Hormonal variation in blood and histomorphological changes in ovaries at different stages of the
estrous cycle were studied in female Wistar rats, which can be used to determine the hormone levels in works
related to hormonal drugs, further ovarian morphology can be used to study changes in ovaries during the
estrous cycle. This study aims to report a rapid and simple method for vaginal cytology using crystal violet
and to report normal hormonal levels and histomorphology of ovaries in various phases of the estrous cycle.

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How to Cite
1.
Begum N, Manipriya K, Begum R, B V. Simple and Rapid Method for Rat Estrous Cycle Identification Using Crystal Violet- Hormonal Consideration. IJAPSR [Internet]. 30Jun.2020 [cited 4Dec.2020];5(04):54-9. Available from: https://sierrajournals.com/index.php/IJAPSR/article/view/1041
Section
Review Article