Development and Validation of Analytical Method for Simultaneous Estimation of Triamterene and Benzthiazide by RP-HPLC

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Gandla Srinija
Sharath Kondra

Abstract

In the process of reversed-phase high-performance liquid chromatography (RP-HPLC), compounds are separated according
to their hydrophobicity. The solute particle from the solvent system binds to the adsorbed hydrophobic ligands coupled
to the sorbent in a hydrophobic manner, which results in their separation. In the present study, Triamterene (TMT) and
Benzthiazide (BZT) were quantified simultaneously using a unique RP-HPLC technology that has been developed and
validated. The technique is carried out on Symmetry C18 (4.6 x 150 mm, 5 m, manufactured: Waters). Methanol: Buffer:
ACN (60:30:10) serves as the mobile phase, injected at a flow rate of 1.0 mL/min, to perform the separation. The study is
carried out with the column fixed at 40°C. The overall run duration is around 6 minutes, and the injecting volume is fixed at
10 μL. According to ICH specifications, the method has been validated for linearity, ruggedness, precision, and specificity,
robustness, and system suitability. The approach is precise and linear for the determination of TMT at 15-75 μg/mL and
BZT at 10-50 μg/mL. Additionally, positive responses are also obtained concerning robustness, intra-day and inter-day
precision, and mean % recovery (100.0% for TMT and 100.3% for BZT). The outcomes show that the approach is appropriate
for regular quality control assessment of commercial tablet dosage forms.

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How to Cite
1.
Srinija G, Kondra S. Development and Validation of Analytical Method for Simultaneous Estimation of Triamterene and Benzthiazide by RP-HPLC. IJAPSR [Internet]. 28Jun.2022 [cited 24Sep.2022];7(03):52-9. Available from: https://sierrajournals.com/index.php/IJAPSR/article/view/1076
Section
Research Article