Method Development and Validation of RP-HPLC Method for the Estimation of Ormeloxifene
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Abstract
A new simple, specific, accurate, precise RP-HPLC method has been developed for the estimation of Ormeloxifene. The chromatographic separation for Ormeloxifene was achieved with mobile phase containing methanol :ACN(70:30 v/v), agilent C18 column (4.6 x150 mm) 5 μ at room temperature and UV detection at 274nm.The compounds were eluted in the isocratic mode at a flow rate of 1ml/min. The retention time of Ormeloxifene was found to be 2.497min. The method was validated according to ICH guideline for linearity, specificity, precision, accuracy, LOD, LOQ and robustness in accordance with ICH guidelines.
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1.
Shivaraj A, Battula S. Method Development and Validation of RP-HPLC Method for the Estimation of Ormeloxifene. IJAPSR [Internet]. 1Jul.2017 [cited 11Jan.2025];2(03):78-2. Available from: https://sierrajournals.com/index.php/IJAPSR/article/view/997
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Review Article
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